pBD21_033
(Plasmid #109150)

• Purpose: pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius
• Depositing Lab: Ron Weiss
• Publication: Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e106. doi: 10.1093/nar/gkz623.

Backbone

• Vector backbone: pL2-S3S1-Kan
• Backbone size w/o insert (bp): 2103
• Total vector size (bp): 5258
• Vector type: Mammalian Expression, Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mRFP
• Species: Synthetic
• Insert Size (bp): 678
• Promoter: pTet

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: Bsai (destroyed during cloning)
• 5′ sequencing primer: GAGACCGTATCACGAGGCAGAATTTCAG
• 3′ sequencing primer: AGTGAGCTGATACCGCTCGC

Gene/Insert 2

• Gene/Insert name: Sirius
• Species: Synthetic
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCTTGTAGTATTGTATAATGTTTTTAAGATCC
• 3′ sequencing primer: GACGAGAGATTGGGTATTAGACC

Resource Information

• Supplemental Documents:
  • pBD21_033.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry