pBabe-Dvl2-GFP
(Plasmid
#109148)
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PurposeStable Dvl2 expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-Puro
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Backbone manufacturerAddgene #1764
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDvl2
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SpeciesH. sapiens (human)
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Entrez GeneDVL2
- Promoter SV40
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPMID: 16263761
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gene Origin - PMID: 16263761
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-Dvl2-GFP was a gift from Mariann Bienz (Addgene plasmid # 109148 ; http://n2t.net/addgene:109148 ; RRID:Addgene_109148) -
For your References section:
Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. Gammons MV, Rutherford TJ, Steinhart Z, Angers S, Bienz M. J Cell Sci. 2016 Oct 15;129(20):3892-3902. 10.1242/jcs.195685 PubMed 27744318