BCL9-BirA*
(Plasmid #109147)

• Purpose: To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells
• Depositing Lab: Mariann Bienz
• Publication: van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife.20882.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Thermofisher
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCL9
• Species: H. sapiens (human)
• Entrez Gene: BCL9 (a.k.a. LGS)
• Promoter: CMV/TetO2
• Tag / Fusion Protein:
  • BirA(R118G) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Wim de Lau and Hans Clevers, Utrecht see PMID: 14612447

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Origin: Wim de Lau and Hans Clevers, Utrecht - see PMID: 14612447