GFP-TCF4
(Plasmid #109144)

• Purpose: For the expression of GFP-TCF in mammalian cells
• Depositing Lab: Mariann Bienz
• Publication: Townsley et al Nat Cell Biol. 2004 Jul;6(7):626-33. doi: 10.1038/ncb1141. Epub 2004 Jun 20.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCF7L2
• Species: H. sapiens (human)
• Entrez Gene: TCF7L2 (a.k.a. TCF-4, TCF4)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PMID: 9065401
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Origin - PMID: 9065401

Note: The TCF7L2 insert contains amino acids 1 through 461 compared to the closest reference sequence, NP_110383.2.