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Addgene

pDEST47-CYFIP2.GFP
(Plasmid #109140)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109140 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEST47
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 7488
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYFIP2
  • Alt name
    cytoplasmic FMR1 interacting protein 2
  • Alt name
    PIR121
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    CYFIP2 (a.k.a. EIEE65, PIR121)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST47-CYFIP2.GFP was a gift from Josef Kittler (Addgene plasmid # 109140 ; http://n2t.net/addgene:109140 ; RRID:Addgene_109140)
  • For your References section:

    The autism and schizophrenia associated gene CYFIP1 is critical for the maintenance of dendritic complexity and the stabilization of mature spines. Pathania M, Davenport EC, Muir J, Sheehan DF, Lopez-Domenech G, Kittler JT. Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16. 10.1038/tp.2014.16 PubMed 24667445