pDEST47-CYFIP2.GFP
(Plasmid
#109140)
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PurposeGFP-tagged CYFIP2 expression construct
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109140 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST47
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Backbone manufacturerInvitrogen
- Total vector size (bp) 7488
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCYFIP2
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Alt namecytoplasmic FMR1 interacting protein 2
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Alt namePIR121
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SpeciesH. sapiens (human)
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GenBank ID
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Entrez GeneCYFIP2 (a.k.a. EIEE65, PIR121)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST47-CYFIP2.GFP was a gift from Josef Kittler (Addgene plasmid # 109140 ; http://n2t.net/addgene:109140 ; RRID:Addgene_109140) -
For your References section:
The autism and schizophrenia associated gene CYFIP1 is critical for the maintenance of dendritic complexity and the stabilization of mature spines. Pathania M, Davenport EC, Muir J, Sheehan DF, Lopez-Domenech G, Kittler JT. Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16. 10.1038/tp.2014.16 PubMed 24667445