pDY-HA1-HA2-neo
(Plasmid #109123)

• Purpose: FR cassette provider to introduce FR cassette to a BAC by recombineering
• Depositing Lab: David Gilbert
• Publication: Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):1810-1820. doi: 10.1093/nar/gkx1265.

Backbone

• Vector backbone: pDY-, pBR322 derivative
• Backbone manufacturer: Michelle Calos
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: HA1
• Alt name: sacB gene on pBACe3.6 vector
• Species: B. subtilis
• Insert Size (bp): 527
• GenBank ID: U80929.2

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AseI (unknown if destroyed)
• 3′ cloning site: SspI (unknown if destroyed)
• 5′ sequencing primer: unavailable

Gene/Insert 2

• Gene/Insert name: HA2
• Alt name: sacB gene on pBACe3.6 vector
• Species: B. subtilis
• Insert Size (bp): 535
• GenBank ID: U80929.2

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NruI (unknown if destroyed)
• 3′ cloning site: NruI (unknown if destroyed)
• 5′ sequencing primer: unavailable

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDY- from Dr. Michelle Calos, Stanford

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.