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Addgene

Rc/CMV cyclin D3
(Plasmid #10912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    CCND3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (destroyed during cloning)
  • 3′ cloning site BstXI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

D3 cloned in as klenow'ed EcoRI fragment at BstXI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rc/CMV cyclin D3 was a gift from Bob Weinberg (Addgene plasmid # 10912 ; http://n2t.net/addgene:10912 ; RRID:Addgene_10912)

  • For your References section:

    Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI, Weinberg RA. Cell. 1992 Sep 18. 70(6):993-1006. 10.1016/0092-8674(92)90249-C PubMed 1388095
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