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pEB5 (mANGPTL8-V5-His lenti)
(Plasmid #109115)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL-cPPT-MCS-IRES-Puro
  • Backbone size w/o insert (bp) 8031
  • Total vector size (bp) 8694
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ANGPTL8
  • Alt name
    Lipasin
  • Alt name
    RIFL
  • Alt name
    betatrophin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    663
  • Entrez Gene
    Angptl8 (a.k.a. EG624219, Gm6484, Rifl)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6x His (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GTGGGAGGTCTATATAAGCAGAGCTCG
  • 3′ sequencing primer CTCACATTGCCAAAAGACG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB5 (mANGPTL8-V5-His lenti) was a gift from Brandon Davies (Addgene plasmid # 109115 ; http://n2t.net/addgene:109115 ; RRID:Addgene_109115)

  • For your References section:

    ANGPTL8 promotes the ability of ANGPTL3 to bind and inhibit lipoprotein lipase. Chi X, Britt EC, Shows HW, Hjelmaas AJ, Shetty SK, Cushing EM, Li W, Dou A, Zhang R, Davies BSJ. Mol Metab. 2017 Oct;6(10):1137-1149. doi: 10.1016/j.molmet.2017.06.014. Epub 2017 Jun 29. 10.1016/j.molmet.2017.06.014 PubMed 29031715
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