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Addgene

NK651
(Plasmid #109107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDSB_0093
  • Vector type
    Choanoflagellate Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTSG_07215 deltaCC
  • Alt name
    pEFL_PTSG_07215deltaCC_mTFP1_3'act
  • Mutation
    Deletes C-terminal coiled-coil from PTSG_07215

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pDSB_0109 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NK651 was a gift from Nicole King (Addgene plasmid # 109107 ; http://n2t.net/addgene:109107 ; RRID:Addgene_109107)

  • For your References section:

    Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins. Booth DS, Szmidt-Middleton H, King N. Mol Biol Cell. 2018 Dec 1;29(25):3026-3038. doi: 10.1091/mbc.E18-08-0514. Epub 2018 Oct 3. 10.1091/mbc.E18-08-0514 PubMed 30281390
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