pCI-Neo HA-hSki
(Plasmid #10910)

• Depositing Lab: Bob Weinberg
• Publication: Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12442-7.

Backbone

• Vector backbone: pCI-neo
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ski
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• GenBank ID: NM_003036.3
• Entrez Gene: SKI (a.k.a. SGS, SKV)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Shunsuke Ishii

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ski was excised from a psp65 vector from S. Ishii. Ski was cloned into pCI-Neo-HA at the SmaI site. But the HA tag was later engineered to adjoin the ATG of Ski directly without intervening vector sequence. So the insert can be cut out with XhoI and HpaI.