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Addgene

pWZL Blast VEGF
(Plasmid #10909)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10909 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pWZL Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VEGF
  • Alt name
    Vascular Endothelial Growth Factor
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Vegfa (a.k.a. L-VEGF, Vegf, Vpf)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Bruce Spiegelman

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Blast VEGF was a gift from Bob Weinberg (Addgene plasmid # 10909)

  • For your References section:

    Ras modulates Myc activity to repress thrombospondin-1 expression and increase tumor angiogenesis. Watnick RS, Cheng YN, Rangarajan A, Ince TA, Weinberg RA. Cancer Cell. 2003 Mar . 3(3):219-31. 10.1016/S1535-6108(03)00030-8 PubMed 12676581
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