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Addgene

NK619
(Plasmid #109089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDSB_0050
  • Vector type
    Choanoflagellate Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Alt name
    pEFL_mCherry
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pDSB_0065 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NK619 was a gift from Nicole King (Addgene plasmid # 109089 ; http://n2t.net/addgene:109089 ; RRID:Addgene_109089)

  • For your References section:

    Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins. Booth DS, Szmidt-Middleton H, King N. Mol Biol Cell. 2018 Dec 1;29(25):3026-3038. doi: 10.1091/mbc.E18-08-0514. Epub 2018 Oct 3. 10.1091/mbc.E18-08-0514 PubMed 30281390
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