pCI-Neo HA-hSnoN
(Plasmid #10908)

• Depositing Lab: Bob Weinberg
• Publication: Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12442-7.

Backbone

• Vector backbone: pCI-neo
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SnoN
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: SKIL (a.k.a. SNO, SnoA, SnoI, SnoN)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Shunsuke Ishii
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SnoN was excised from a psp65 vector from S. Ishii. SnoN was cloned into pCI-Neo-HA at the SmaI site. But the HA tag was later engineered to adjoin the ATG of SnoN directly without intervening vector sequence. So the insert can be cut out with XhoI and HpaI.