pstop
(Plasmid
#109066)
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Purpose(Empty Backbone) inserts a polyA site to end transcription of a targetted gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSTOP
- Backbone size (bp) 3794
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pstop was a gift from Eric Lander (Addgene plasmid # 109066 ; http://n2t.net/addgene:109066 ; RRID:Addgene_109066) -
For your References section:
Local regulation of gene expression by lncRNA promoters, transcription and splicing. Engreitz JM, Haines JE, Perez EM, Munson G, Chen J, Kane M, McDonel PE, Guttman M, Lander ES. Nature. 2016 Nov 17;539(7629):452-455. doi: 10.1038/nature20149. Epub 2016 Oct 26. 10.1038/nature20149 PubMed 27783602
