siSlug3
(Plasmid #10905)

• Depositing Lab: Bob Weinberg
• Publication: Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: siSlug
• Alt name: Slug siRNA
• Alt name: Slug
• Species: H. sapiens (human)
• Insert Size (bp): 60
• Entrez Gene: SNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sequence: 5'-CAG ACC CAT TCT GAT GTA AAG-3'