PAX6 TALEN L
(Plasmid #109034)

• Purpose: Knock in H2B-GFP into the human PAX6 locus
• Depositing Lab: Lorenz Studer
• Publication: Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410.e7. doi: 10.1016/j.stem.2017.08.015.

Backbone

• Vector backbone: pTALEN
• Backbone size w/o insert (bp): 7961
• Total vector size (bp): 8118
• Vector type: Mammalian Expression, TALEN
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pax6 TALEN L
• Species: Synthetic
• Insert Size (bp): 3012
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3x FLAG (N terminal on insert)
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid kit used for building TALENs and TALE-TFs was a gift from Feng Zhang (Addgene kit # 1000000019)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pair with Pax6 TALEN R