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pLenti-X1-Neo-GFP-FAM134B-LIRmut
(Plasmid #109027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-X1-Neo
  • Backbone manufacturer
    Michael Rape
  • Backbone size w/o insert (bp) 9005
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418) ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FAM134B-LIRmut
  • Alt name
    RETREG1-LIRmut
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2253
  • Mutation
    DDFELL to DDAEAL aa 453-458 to interrupt FAM134B interaction with LC3 family proteins
  • Entrez Gene
    RETREG1 (a.k.a. FAM134B, JK-1, JK1)
  • Promoter EIF-1a promoter
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGCCTCAGACAGTGGTTCAA
  • 3′ sequencing primer agcagcgtatccacatagcgt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-X1-Neo-GFP-FAM134B-LIRmut was a gift from Jacob Corn (Addgene plasmid # 109027 ; http://n2t.net/addgene:109027 ; RRID:Addgene_109027)

  • For your References section:

    Atlastins remodel the endoplasmic reticulum for selective autophagy. Liang JR, Lingeman E, Ahmed S, Corn JE. J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185. 10.1083/jcb.201804185 PubMed 30143524
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