pDM1515
(Plasmid #109000)

• Purpose: (Empty Backbone) safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging
• Depositing Lab: Rob Kay
• Publication: Paschke et al PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018.

Backbone

• Vector backbone: pDM1501
• Backbone size (bp): 6154
• Vector type: safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging
• Selectable markers: Hygromycin
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.