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pJCSUP35(123-254)
(Plasmid #1090)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC45
  • Backbone manufacturer
    J. Clos and Brandau, 1994
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SUP35
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    393
  • Mutation
    amino acids 123 - 254
  • GenBank ID
    AY553990
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ter-Avanesyan et al., 1993

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJCSUP35(123-254) was a gift from Susan Lindquist (Addgene plasmid # 1090 ; http://n2t.net/addgene:1090 ; RRID:Addgene_1090)

  • For your References section:

    Self-seeded fibers formed by Sup35, the protein determinant of [PSI+], a heritable prion-like factor of S. cerevisiae. Glover JR, Kowal AS, Schirmer EC, Patino MM, Liu JJ, Lindquist S. Cell 1997 May 30;89(5):811-9. 10.1016/S0092-8674(00)80264-0 PubMed 9182769
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