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7861 pRS305-pUG72-HSC82out
(Plasmid #10899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    LEU2, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HSC82 URA3
  • Alt name
    HSC82
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4200
  • Entrez Gene
    HSC82 (a.k.a. YMR186W, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Yeast integrating plasmid. URA3 flanked by loxP sites. HSC82 outside of the loxP sites.
Insert from plasmid 7852 pUG72-HSC82out.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7861 pRS305-pUG72-HSC82out was a gift from Susan Lindquist (Addgene plasmid # 10899 ; http://n2t.net/addgene:10899 ; RRID:Addgene_10899)

  • For your References section:

    Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi. Cowen LE, Lindquist S. Science. 2005 Sep 30. 309(5744):2185-9. 10.1126/science.1118370 PubMed 16195452
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