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Addgene

pDM1082
(Plasmid #108982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDM304
  • Backbone size w/o insert (bp) 2071
  • Vector type
    Cre/Lox ; Dictyostelium discoideum knockout vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Knockout cassette G418
  • Insert Size (bp)
    1332
  • Promoter coaA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDM1082 was a gift from Rob Kay (Addgene plasmid # 108982 ; http://n2t.net/addgene:108982 ; RRID:Addgene_108982)

  • For your References section:

    Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM. PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018. 10.1371/journal.pone.0196809 PubMed 29847546
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