pVIM-BC2T
(Plasmid
#108967)
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Purposeexpresses vimentin-BC2-tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVimentin
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Alt nameVim
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- BC2T (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVIM-BC2T was a gift from Ulrich Rothbauer (Addgene plasmid # 108967 ; http://n2t.net/addgene:108967 ; RRID:Addgene_108967) -
For your References section:
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging. Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhofer M, Schmees C, Vojnovic I, Pisak-Lukats B, Endesfelder U, Rothbauer U. Nat Commun. 2018 Mar 2;9(1):930. doi: 10.1038/s41467-018-03191-2. 10.1038/s41467-018-03191-2 [pii] PubMed 29500346
