pDJSVT86
(Plasmid
#108965)
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Purpose(Empty Backbone) Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-16b
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Backbone manufacturerEMD Millipore
- Backbone size (bp) 5711
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Modifications to backboneRemoved DNA between NcoI and XhoI sites and replaced with a new sequence.
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- OmpA 1-27, 6His (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDJSVT86 was a gift from Daniel Slade (Addgene plasmid # 108965 ; http://n2t.net/addgene:108965 ; RRID:Addgene_108965) -
For your References section:
A chemical and biological toolbox for Type Vd secretion: Characterization of the phospholipase A1 autotransporter FplA from Fusobacterium nucleatum. Casasanta MA, Yoo CC, Smith HB, Duncan AJ, Cochrane K, Varano AC, Allen-Vercoe E, Slade DJ. J Biol Chem. 2017 Dec 8;292(49):20240-20254. doi: 10.1074/jbc.M117.819144. Epub 2017 Oct 11. 10.1074/jbc.M117.819144 PubMed 29021252
Map uploaded by the depositor.
