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Purposeheterologous expression of GDH of B. megaterium in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlucose dehydrogenase IV (GDH IV)
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SpeciesBacillus megaterium
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Tag
/ Fusion Protein
- His6-Tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Wild Type Gene
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-28a(+)-GDH was a gift from Frank Schulz (Addgene plasmid # 108954 ; http://n2t.net/addgene:108954 ; RRID:Addgene_108954) -
For your References section:
An in vitro Biosynthesis of Sesquiterpenes starting from Acetic Acid. Schulz F, Dirkmann M, Nowack J. Chembiochem. 2018 Aug 7. doi: 10.1002/cbic.201800128. 10.1002/cbic.201800128 PubMed 30085399
