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Addgene

7851 pUG72-HSC82in
(Plasmid #10894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUG72
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HSC82
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2700
  • Entrez Gene
    HSC82 (a.k.a. YMR186W, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HSC82 and URA3 are flanked by Lox sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7851 pUG72-HSC82in was a gift from Susan Lindquist (Addgene plasmid # 10894 ; http://n2t.net/addgene:10894 ; RRID:Addgene_10894)

  • For your References section:

    Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi. Cowen LE, Lindquist S. Science. 2005 Sep 30. 309(5744):2185-9. 10.1126/science.1118370 PubMed 16195452
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