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HA-mRIN2 dRHVPS9/PINCO
(Plasmid #108939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PINCO
  • Backbone manufacturer
    Pellicci's lab
  • Backbone size w/o insert (bp) 12889
  • Total vector size (bp) 14641
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    EGFP; Puro marker is outside the LTRs and will not be packaged into virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ras and Rab interactor 2
  • Alt name
    Rin2
  • Alt name
    RASSF4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1752
  • Mutation
    Deletion of RHVPS9 domain (aa 455-738)
  • GenBank ID
    74030
  • Entrez Gene
    Rin2 (a.k.a. 2010003K16Rik, 4632403N06Rik, AW821980, RASSF4)
  • Promoter LTR
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-mRIN2 dRHVPS9/PINCO was a gift from Guido Serini (Addgene plasmid # 108939 ; http://n2t.net/addgene:108939 ; RRID:Addgene_108939)

  • For your References section:

    The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and morphogenesis via active integrin endocytosis and Rac signaling. Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G. Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 2012 Jul 24. 10.1038/cr.2012.110 PubMed 22825554
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