pSEMS-CV SUy-Halo7Tag
(Plasmid #108933)

• Purpose: Selflabeling tag at ATP Synthase
• Depositing Lab: Karin Busch
• Publication: Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16. doi: 10.1242/jcs.108852. Epub 2012 Oct 4.

Backbone

• Vector backbone: pSEMS(26m)
• Backbone manufacturer: Covalys, NEB
• Backbone size w/o insert (bp): 5325
• Total vector size (bp): 7026
• Modifications to backbone: original snap-Tag substituted by Halo7-Tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATP synthase F1 subunit gamma
• Alt name: ATP5F1C
• Species: H. sapiens (human)
• Insert Size (bp): 891
• GenBank ID: NM_001001973.2
• Entrez Gene: ATP5F1C (a.k.a. ATP5C, ATP5C1, ATP5CL1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Halo7 tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ATCGATATGTTCTCTCGCGCGGGTGTCGCTGGGCTGTCGGCC
• 3′ sequencing primer: GAAATTATCTCTGGTGCTGCAGCTCTGGCGGAATTCCG

Resource Information

• Supplemental Documents:
  • CV-Halo7.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry