pIRESh-GFPII-S-Dlk1
(Plasmid
#108931)
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PurposeGFP-tagged vector expressing secreted isoform of Dlk1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRESh-GFPII
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDlk1
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Alt nameNotch ligand Delta-like homologue 1
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SpeciesM. musculus (mouse)
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Mutationcontains sequences from full length Dlk1A up to the juxtamembrane cleavage domain (amino acid 303)
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Entrez GeneDlk1 (a.k.a. DLK-1, DlkI, FA1, Ly107, Peg9, SCP1, ZOG, pG2, pref-1)
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Tag
/ Fusion Protein
- GFPII
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRESh-GFPII-S-Dlk1 was a gift from Anne Ferguson-Smith (Addgene plasmid # 108931 ; http://n2t.net/addgene:108931 ; RRID:Addgene_108931) -
For your References section:
Postnatal loss of Dlk1 imprinting in stem cells and niche astrocytes regulates neurogenesis. Ferron SR, Charalambous M, Radford E, McEwen K, Wildner H, Hind E, Morante-Redolat JM, Laborda J, Guillemot F, Bauer SR, Farinas I, Ferguson-Smith AC. Nature. 2011 Jul 20;475(7356):381-5. doi: 10.1038/nature10229. 10.1038/nature10229 PubMed 21776083