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pIRESh-GFPII-MB-Dlk1
(Plasmid #108930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESh-GFPII
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dlk1
  • Alt name
    Notch ligand Delta-like homologue 1
  • Species
    M. musculus (mouse)
  • Mutation
    lacks coding sequence for the juxtamembrane motif, amino acids 231-305, that is the substrate for cleavage and so represents Dlk1C
  • Entrez Gene
    Dlk1 (a.k.a. DLK-1, DlkI, FA1, Ly107, Peg9, SCP1, ZOG, pG2, pref-1)
  • Tag / Fusion Protein
    • GFPII

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESh-GFPII-MB-Dlk1 was a gift from Anne Ferguson-Smith (Addgene plasmid # 108930 ; http://n2t.net/addgene:108930 ; RRID:Addgene_108930)

  • For your References section:

    Postnatal loss of Dlk1 imprinting in stem cells and niche astrocytes regulates neurogenesis. Ferron SR, Charalambous M, Radford E, McEwen K, Wildner H, Hind E, Morante-Redolat JM, Laborda J, Guillemot F, Bauer SR, Farinas I, Ferguson-Smith AC. Nature. 2011 Jul 20;475(7356):381-5. doi: 10.1038/nature10229. 10.1038/nature10229 PubMed 21776083
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