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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-neo
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelarge T and small T antigen
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Alt namelarge T genomic DNA
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Alt nameLTg
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Speciessimian virus
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Insert Size (bp)2100
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Entrez GeneSV40gp6 (a.k.a. SV40gp6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-neo largeTgenomic was a gift from William Hahn (Addgene plasmid # 10891 ; http://n2t.net/addgene:10891 ; RRID:Addgene_10891)
Map uploaded by the depositor.