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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWZL Neo
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep110alpha
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Alt namePI3K p110
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Alt namePIK3CA
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SpeciesB. taurus (bovine)
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Insert Size (bp)3600
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GenBank IDNM_174574
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Entrez GenePIK3CA
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Tags
/ Fusion Proteins
- Myr (N terminal on insert)
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EagI (unknown if destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWZL-neo Myr-FLAG-p110alpha was a gift from William Hahn (Addgene plasmid # 10890 ; http://n2t.net/addgene:10890 ; RRID:Addgene_10890) -
For your References section:
Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM, Hahn WC. Cancer Cell. 2003 May . 3(5):483-95. 10.1016/S1535-6108(03)00088-6 PubMed 12781366
Map uploaded by the depositor.
