p3E-csGBP
(Plasmid
#108891)
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PurposeMultisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR P2R-p3
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Backbone manufacturerInvitrogen
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Vector typeMultisite gateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecsGBP
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SpeciesSynthetic
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E-csGBP was a gift from Rob Parton (Addgene plasmid # 108891 ; http://n2t.net/addgene:108891 ; RRID:Addgene_108891) -
For your References section:
Ultrastructural localisation of protein interactions using conditionally stable nanobodies. Ariotti N, Rae J, Giles N, Martel N, Sierecki E, Gambin Y, Hall TE, Parton RG. PLoS Biol. 2018 Apr 5;16(4):e2005473. doi: 10.1371/journal.pbio.2005473. eCollection 2018 Apr. 10.1371/journal.pbio.2005473 PubMed 29621251