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Addgene

pME-H2B
(Plasmid #108883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR 221
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Multisite gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B (human amino acid sequence but different codon usage)
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    human amino acid sequence but different codon usage
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-H2B was a gift from Rob Parton (Addgene plasmid # 108883 ; http://n2t.net/addgene:108883 ; RRID:Addgene_108883)

  • For your References section:

    Ultrastructural localisation of protein interactions using conditionally stable nanobodies. Ariotti N, Rae J, Giles N, Martel N, Sierecki E, Gambin Y, Hall TE, Parton RG. PLoS Biol. 2018 Apr 5;16(4):e2005473. doi: 10.1371/journal.pbio.2005473. eCollection 2018 Apr. 10.1371/journal.pbio.2005473 PubMed 29621251
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