pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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GenBank

SnapGene

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> Addgene NGS Result TGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTT TTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTG TTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGT CTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACC TCCTGTCCAACAATGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGA AATGCCCTCTGATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGGT CAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGTGAAAGTAC AAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGT AAAAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAACTAG AGATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTA GAACTGTTTGTATTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTG TAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGCTCTTTTGG GGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGT CTTGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGA TGGGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGC TTCAGGGGTGTCGCTTGGTTATTTCCAAAAATCAAATTAATTTTATTAAACTATTAGAACGAGCATGTTT TGTCTATATGCTACAGAAGATAAAAAATAATAGGAGTTAACAGTTATAAAACAACACACTTTGTTTCTAT TGATTGTTGACCACACTGGGGTCTCATTAAGTTAGATTAAAGACACACTAACTGGGTCAAAAGCAGCAGA TTGATTTCATAGCACCAGGGTAAACTTTCTAACACTTTTACGGCAATCATATACATTAAAATTAAATACA GACCACGACTGAACAAGGAGGATGATCTCCAATATTAAACAAAGAGACTTGTGCCTATTTCTCTGAGGGT AAACATGACCTCTCAAGTTAGCAAGTTGGTTTTAACACTACAAAAATAGTTAAGACTGCAATCCCAGAAT AAAGTATTGGTTTTAACCAATCAATATAGTACAGTAAACATCCATTTGTTTTGTTGAAACGTTAAACAAA TCTGACCAAAGCTATTAGCTTATATAAAACAGGTTTGCCTTCTATGTAGCTGAAAACACCACAGGCCCGA TTTTGCTACTGTGTAAAACATTTCAGCAAGATTTTTTTATTGCATTTTTTTTTACTGAATCGTTCAAACA TTTTATCATTTTAGTTTGTTCATTCATTGCAACTGGAAAAACAACACATCACACAACCGCACATATTTCA GCAATAAGTACAATAAAACACTCAAATAAAAAAAACATTTTAAATCTCTTTGTATTTTTGACCGCTGTTT CGCGTAATTTCACGGTAAAACTCTGGAAATCTCCACTACATTCCTCTCAGCGGCTCCTCTCAATGACAGC TGAAGAAGTGACGCGGCTGCCTGCTGTGTTTTGATTGGTCGAATTCACTGGAGGCTTCCAGAACAGTGTA GAGTCTGAACGGGTGCGCGCTCTGCTGTATTTAAAGGGCGAAAGAGAGACCGCAGAGAAACTCAACCGAA GAGAAGCGACTTGACAAAGAAGAAAAGAGCAGCCTGACAGGACTTTTTCCCCGACGAGGTGTTTATTCGC TCTATTTAAGAATCTACTGTAAGGTAAGTCTCAATATATTGTACTCTATTGGCTAATCAGAATTATATAG AGATTATATGTACTTAATGTCAAAAAATCAACTTTGTATATGTAATCTTTTTACATGTGGACTGCCTATG TTCATCTTATTTTAGGTCTACTAGAAAATTATATTTCCCGTTTTCACAATAAGGATTTTTAAAAAAAGCA ATGAACAGACGGGCATTTACTTTATGTTGCTGACATTATTTTATATGAGCATAATAACCATAAATACTAG CAAATGTCCTAAATGAATTTGTGTTAATGTTGTCTACAAAAGAAAATTAGCGTTTTACTTGTACAACTAA TAATAACTTGGTTATTAAGAGAATTTCACTTGTTGACTAGAAAAATCCTTTCATAATGAAACAATTGCAC CATAAATTGTATAAATATAAAATTAATTCTAATTGTTTTTTTTTCCTGCCAAGTTTGTACAAAAAAGCAG GCTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCC CCCTCGAGGTCGACGCCACCATGGCTTCTCCACCTAAGAAGAAGAGAAAGGTGGGAAGCATGGATAAGAA GTATAGCATCGGCCTGGATATTGGAACTAACTCCGTGGGTTGGGCAGTGATTACAGACGACTACAAGGTC CCTAGCAAGAAATTTAAGGTGCTGGGTAACACCGACAGGCACAGCATCAAGAAAAATCTGATTGGAGCCC TGCTGTTCGGTTCTGGAGAGACTGCCGAAGCAACACGCCTGAAAAGAACAGCAAGAAGGCGCTATACCAG AAGGAAGAATAGAATCTGTTACCTGCAGGAGATTTTCTCTAACGAAATGGCTAAGGTGGACGATTCATTC TTTCATAGGCTGGAGGAAAGTTTCCTGGTCGAGGAAGATAAGAAACACGAGCGCCATCCTATCTTTGGAA ACATTGTGGACGAGGTCGCCTATCACGAAAAATACCCAACCATCTATCATCTGCGCAAGAAACTGGCTGA CTCTACTGATAAAGCCGACCTGAGACTGATCTATCTGGCTCTGGCCCACATGATTAAGTTCAGGGGTCAT TTTCTGATCGAGGGCGATCTGAACCCCGACAATTCCGATGTGGACAAGCTGTTCATCCAGCTGGTCCAGA TTTACAATCAGCTGTTTGAGGAAAACCCTATTAATGCTTCCAGAGTGGACGCAAAAGCTATCCTGTCAGC CAGGCTGTCCAAGTCACGCAGACTGGAGAACCTGATTGCACAGCTGCCCGGAGAAAAGAGGAACGGTCTG TTTGGAAATCTGATCGCTCTGAGTCTGGGCCTGACTCCTAACTTCAAAAGCAATTTTGATCTGGCTGAGG ACGCCAAACTGCAGCTGTCAAAGGACACATATGACGATGACCTGGATAACCTGCTGGCACAGATCGGAGA TCAGTACGCTGACCTGTTCCTGGCTGCCAAAAATCTGTCCGACGCAATCCTGCTGTCAGATATTCTGAGA GTGAACAGCGAGATTACAAAAGCACCTCTGAGTGCCAGCATGATCAAGAGATATGACGAGCACCATCAGG ATCTGACCCTGCTGAAGGCTCTGGTCAGGCAGCAGCTGCCAGAGAAGTACAAGGAAATTTTCTTTGATCA GTCCAAGAACGGCTACGCCGGTTATATCGACGGAGGCGCATCACAGGAGGAATTCTACAAGTTTATCAAA CCTATTCTGGAGAAGATGGACGGAACTGAGGAACTGCTGGTGAAACTGAATAGAGAGGACCTGCTGAGGA AGCAGCGCACATTTGATAACGGTTCCATCCCACACCAGATTCATCTGGGAGAGCTGCACGCTATCCTGAG GCGCCAGGAAGACTTCTACCCCTTTCTGAAAGATAACCGCGAGAAGATCGAAAAAATTCTGACCTTCAGA ATCCCTTACTATGTGGGTCCACTGGCTCGCGGAAACAGCAGATTTGCCTGGATGACTCGCAAATCCGAGG AAACCATTACTCCTTGGAACTTCGAGGAAGTGGTCGATAAGGGCGCCTCTGCACAGTCCTTCATCGAGAG AATGACTAATTTTGACAAAAACCTGCCCAATGAGAAAGTGCTGCCTAAGCACTCCCTGCTGTACGAGTAT TTCACTGTCTATAACGAACTGACAAAGGTGAAATACGTCACCGAGGGCATGAGAAAGCCAGCCTTCCTGT CAGGAGAGCAGAAGAAAGCAATCGTGGATCTGCTGTTTAAAACCAACAGGAAAGTGACTGTCAAGCAGCT GAAGGAGGACTACTTCAAGAAAATTGAATGCTTCGATTCCGTGGAGATCAGCGGAGTCGAAGACAGATTT AACGCAAGCCTGGGCGCTTACCACGATCTGCTGAAGATCATTAAGGATAAAGACTTCCTGGACAACGAGG AAAATGAGGATATCCTGGAAGACATTGTGCTGACACTGACCCTGTTTGAGGACAGAGGAATGATCGAGGA AAGACTGAAAACCTATGCTCATCTGTTCGATGACAAGGTGATGAAACAGCTGAAGAGAAGGCGCTACACT GGCTGGGGTAGACTGAGCAGGAAGCTGATCAACGGCATTAGGGATAAACAGTCAGGAAAGACAATCCTGG ACTTTCTGAAAAGTGATGGCTTCGCCAACCGCAATTTTATGCAGCTGATTCACGATGACAGTCTGACCTT CAAAGAGGACATCCAGAAGGCTCAGGTGTCTGGACAGGGCCACTCCCTGCATGAGCAGATTGCAAACCTG GCTGGAAGCCCAGCCATCAAGAAAGGCATTCTGCAGACAGTGAAAATCGTCGATGAGCTGGTGAAAGTCA TGGGCCATAAGCCCGAAAACATCGTGATTGAGATGGCTCGCGAAAATCAGACAACCCAGAAGGGTCAGAA GAACAGTAGAGAGAGGATGAAAAGAATCGAGGAAGGCATTAAGGAGCTGGGTAGCCAGATCCTGAAAGAG CACCCAGTGGAAAACACACAGCTGCAGAATGAGAAGCTGTATCTGTACTATCTGCAGAATGGAAGAGATA TGTACGTGGACCAGGAGCTGGATATTAACAGGCTGTCTGATTACGACGTGGATCATATCGTCCCCCAGAG TTTCATCAAAGATGACAGCATTGACAACAAGGTGCTGACCAGGTCCGACAAAAACAGAGGAAAATCAGAT AATGTCCCTAGTGAGGAAGTGGTCAAGAAAATGAAGAACTACTGGAGACAGCTGCTGAATGCCAAACTGA TCACTCAGAGGAAGTTTGATAACCTGACAAAAGCAGAGCGCGGTGGACTGTCAGAACTGGACAAAGCTGG ATTCATCAAGAGGCAGCTGGTGGAAACACGCCAGATCACTAAACACGTCGCACAGATTCTGGATAGTCGC ATGAACACAAAGTACGATGAGAATGACAAACTGATCAGAGAAGTGAAGGTCATTACCCTGAAGAGTAAAC TGGTCAGCGACTTTAGGAAAGATTTCCAGTTTTATAAGGTCCGCGAGATTAACAATTATCACCATGCCCA TGACGCATACCTGAACGCCGTGGTCGGTACCGCACTGATCAAGAAATACCCAAAACTGGAGAGCGAATTC GTGTACGGAGACTATAAGGTGTACGATGTCAGAAAAATGATCGCCAAGTCCGAGCAGGAAATTGGAAAAG CTACTGCCAAGTATTTCTTTTACTCAAACATCATGAATTTCTTTAAGACAGAGATCACCCTGGCCAATGG AGAAATCCGCAAAAGGCCCCTGATTGAGACAAACGGAGAGACAGGCGAAATCGTGTGGGACAAAGGCAGA GATTTTGCAACCGTGAGGAAGGTCCTGAGCATGCCTCAAGTGAATATCGTCAAGAAAACTGAGGTGCAGA CAGGCGGTTTCTCAAAAGAAAGTATTCTGCCAAAACGCAACTCTGATAAGCTGATCGCTAGAAAGAAAGA CTGGGACCCTAAGAAGTATGGAGGCTTTGACTCTCCCACTGTGGCATACTCCGTCCTGGTGGTCGCTAAG GTGGAGAAGGGCAAAAGCAAGAAACTGAAATCTGTCAAGGAGCTGCTGGGTATCACAATTATGGAGAGAA GCTCTTTCGAGAAGAACCCAATCGATTTTCTGGAGGCCAAAGGTTATAAGGAAGTGAAGAAAGACCTGAT CATTAAACTGCCCAAGTACAGTCTGTTTGAGCTGGAAAACGGCAGGAAACGCATGCTGGCAAGCGCTGGA GAGCTGCAGAAAGGCAATGAACTGGCCCTGCCTTCTAAGTACGTGAACTTCCTGTATCTGGCAAGCCACT ACGAGAAGCTGAAAGGATCTCCAGAGGATAACGAACAGAAACAGCTGTTTGTGGAGCAGCACAAGCATTA TCTGGACGAGATCATTGAACAGATTAGCGAGTTCTCTAAAAGAGTGATCCTGGCCGACGCAAATCTGGAT AAGGTCCTGTCTGCTTACAACAAACACAGAGATAAGCCCATCAGGGAGCAGGCCGAAAATATCATTCATC TGTTCACTCTGACAAACCTGGGCGCACCTGCAGCTTTCAAGTACTTCGACACTACAATCGATAGAAAGAG GTACACCTCCACTAAGGAGGTGCTGGACGCTACACTGATCCATCAGAGTATTACCGGCCTGTACGAAACA AGGATTGACCTGTCTCAGCTGGGTGGCGACTCACCGGTGGGATCCCCTAAGAAGAAGAGAAAGGTGAGGT CTGGTGGCGGAGAGGGCAGAGGCAGTCTGCTGACATGCGGTGACGTGGAAGAGAATCCCGGCCCTTCTAG AATGGTGAGCAAGGGCGAAGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTA AACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGT TCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCA GTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTAC GTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGG GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCA CAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAG GTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACA CCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAA AGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGC ATGGACGAGCTGTACAAGTAAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGG TTAATTGCGCGCTTGGCGTAATCATGGTCATAGCACCCAGCTTTCTTGTACAAAGTGGGGGATCCAGACA TGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGA AATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTCCAACTTTATTATACATAGTTGAT AATTCACTGGCCGTCGTTTTACGGTACCGCACACTATTAATGGTACGAACTTTAAAAAAAGCACCGACTC GGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCATAGCTC TTAAACTAGTCGACTGTCGTGCAGTCGAACTGAGAGCCGGAAGAAGAGCTATATATACCAGGGAATTCTG GGTATGTTTCTTACTGGTGGTAAGTAGATACACCCCTTATTTAATTAGCTTCCAACAAATTTGAATGTAT ACACTGGTGAGATAGACCTGTAAACTCTTATGATCTGCAAAATTGCAAGCCAATGTTTTTGTGGAAGGTT TATTTTGAGTATTTCCTCAGCTGTAGACCACATGTAAAAAAGGTTCCTCTCTGCTAGCTGTGATTGGATG TTTCGGTGTGACAGTGAAGAACCCGTGGCCACAATGTTCCACACCCTCCCCCACAGACATGCGTAGAATG TTTCCCCCTCAACGATGACGAGAACAAAAGGTTGAAAGACCAGCAAATGCTCCTTTCACAACCACCAAAT CACAGGTTTAAAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTAAAC TTGCTATGCTGTTTCCAGCATAGCTCTTAAACTGACAGCAGATACTGCTGTCGAACTAGGAGCCTGGAGA ACTGCTATATAAACCCGGGACTTCTGGGTATGTTTCTGCCATGTGGTAAGTGGATAAACCTACTTATTCA ACTCATTTACCTCAAACATGACAGTAAACCTCTTCGGGGCAAACCTGCAAACGCTTAAAACCTTCAAAGT TTGAAACCAGCAGTTTTGTAGAGGTTTACCGTAGTATTTTTGAACTGTAAAGCATATGCAGAGGAAGCTG GTTTTAGGCTTGTGTGATTGGATGAGTTCTTCTGTGACGTAACTGATCTTTTGTCCTCGTGTCCTTCGCC TTCCCCCACAGGCATGCGCGGACCTTCCCCCGCCGCAGACCAGAGGACAACATGCTCAGACGCCAGCAAA CGGCCCATCGAAACATTCACAGACTCTCGGATTGGTTTAAAAAAAGCACCGACTCGGTGCCACTTTTTCA AGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCATAGCTCTTAAACTACTGCATG ATATCTCTGTCGAACTGGGAGTCTGGAGGACGGCTATATATACCAGTGACTTCTGGGTATGTGTTTCATG TGTGGTATATGGCTTACTTACTTATTCAACTCATTTACACTTACATGTGTCAGTAAATCACTGTGTAGTA GTAAACCTGTAAACCCTTAAATTCTTTAAAATTTGAAAACCAGCAGTTTATGTAGGTTTACCGTAGAGTT TTGGAGCTGTAAGCGTATGCGAAGGAAACAGGCCGTTTCTTTAGAGTGATTGGATGAGTTCCTGAACTTC CTGTGTGACATCGAGGAGCTTTTGTTCACGTGTCCTTCTCCTCCCCCCACAGGCATGCGCAGATCCCCCC TGCCTTGGATAAGAGCACAAAACACTCCGACAAAAGGAAGCTGCTCTCACTAGAGCAGAGCCCATGAAAA CCCAGACAGACACGTGGAGGGTAATGAGTTTAAAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGAT AACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCATAGCTCTTAAACTAGTCGTAGCGACTGT AGTCGAACCAAGAGCTGGAGGGAGAGCTATATATACCAGGGACTTCTGGGTATGTTTTTGGGAGGTGGTG AGTGACTAAACCACTTATTCAGCTCCCTTAGATCAAGTCTGACCCTATATCATGGTGACATAAACCTGCA AACTGATAAAACCTGAAGGATCTCAAATCCAGAGTTTGTGTGAGGGATTACCGTGGTAATTTTCAAACTG TAAAGCATATGCAAAATTATCTGGTCTTGGCTTGAGTGATTGGGTGTCTCGGTGTGATGCAGGGACGTTT TCAGTGACGTGTCCTTCTCCCTCCCCCACAGGCATGCGCAGAACATTTCCCCCCTCCTTGAAGACCAGAA CAAAAGACGCCGAGAGCAGGAAACTCGTCTTACTGAATGACCGAGGCTGGAGAAACGCTGAGAGACTTGA CTAAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATG CTGTTTCCAGCATAGCTCTTAAACTACTCGATGTCTATGCTCTCGAACCAAGAGCTGGAGGGAGAGCTAT ATATACCAGGGACTTCTGGGTATGTTTTTGGGAGGTGGTGAGTGACTAAACCACTTATTCAGCTCCCTTA GATCAAGTCTGACCCTATATCATGGTGACATAAACCTGCAAACTGATAAAACCTGAAGGATCTCAAATCC AGAGTTTGTGTGAGGGATTACCGTGGTAATTTTCAAACTGTAAAGCATATGCAAAATTATCTGGTCTTGG CTTGAGTGATTGGGTGTCTCGGTGTGATGCAGGGACGTTTTCAGTGACGTGTCCTTCTCCCTCCCCCACA GGCATGCGCAGAACATTTCCCCCCTCCTTGAAGACCAGAACAAAAGACGCCGAGAGCAGGAAACTCGTCT TACTGAATGACCGAGGCTGGAGAAACGCTGAGAGACTTACTGATGCATTTCATTTAATGTATCGATGATG ATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTT TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACC TCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTGCGAAGATACGGCCACGGGTGCTCT TGATCCTGTGGCTGATTTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCT TCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGTAATAGATGTG ACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCA TAGGCAGAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGG GAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAATAAAATTGTAAGGAGTAAAA AGTACTTTTTTTTCTAAAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTA AAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTACTTTACACCTC TGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTG GAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT GGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAA CCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCT TCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAA AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCAT CACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTT GGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGCATTTTGGTC ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTG TCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCA TCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACC AGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGC ATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTA CATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTT GGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGA TGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAA ACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGT GCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAA ATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTA TTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA ATAGGGGTTCCGCGCACATTTCCCCGAAAAG

pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA Sequences (1)

Full Sequences from Addgene (1)

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