pLJM1 EGFP-Tau
(Plasmid #108868)

• Purpose: Expresses EGFP linked to human Tau
• Depositing Lab: Gabriele Kaminski Schierle
• Publication: Bacterial expression plasmids and lentivirus expression vectors for tau and alpha-synuclein (unpublished)

Backbone

• Vector backbone: pLJM1
• Backbone size w/o insert (bp): 7317
• Modifications to backbone: EGFP-Tau cloned from Addgene plasmid #46904 and inserted into Addgene plasmid #19319.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tau
• Alt name: MAPT
• Species: H. sapiens (human)
• Insert Size (bp): 1884
• Mutation: human four-repeat tau lacking the N-terminal sequences (4R0N) and containing exons 1, 4 and 5, 7, and 9–13, intron 13, and exon 14
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: BstB1 (unknown if destroyed)
• 5′ sequencing primer: CMV_F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry