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Addgene

pET29b Tau Cys
(Plasmid #108867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    EMB Biosciences
  • Backbone size w/o insert (bp) 6742
  • Modifications to backbone
    Addgene plasmid #16316 mutated at I260C C291A C322A to allow maleimide dye labelling.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tau
  • Alt name
    Tau T40
  • Alt name
    tau transcript varient 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Mutation
    I260C C291A C322A to allow maleimide dye labelling
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b Tau Cys was a gift from Gabriele Kaminski Schierle (Addgene plasmid # 108867 ; http://n2t.net/addgene:108867 ; RRID:Addgene_108867)
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