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Addgene

pT7-7 aSyn C141
(Plasmid #108866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7-7
  • Backbone size w/o insert (bp) 4238
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha synuclein
  • Alt name
    NACP
  • Alt name
    SNCA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    426
  • Mutation
    C141 mutation to allow C-terminal maleimide dye labelling
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.10.04.510646 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-7 aSyn C141 was a gift from Gabriele Kaminski Schierle (Addgene plasmid # 108866 ; http://n2t.net/addgene:108866 ; RRID:Addgene_108866)

  • For your References section:

    alpha-synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased uptake into neurons. Stephens AD, Fernandez Villegas A, Chung CW, Vanderpoorten O, Pinotsi D, Mela I, Ward E, McCoy TM, Cubitt R, Routh AF, Kaminski CF, Kaminski Schierle GS. bioRxiv 2022.10.04.510646 10.1101/2022.10.04.510646
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