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pYPQ230-RVR
(Plasmid #108861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible Cpf1 entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
  • Species
    Lachnospiraceae bacterium ND2006
  • Mutation
    G548R, K554V and Y558R, Cpf1 is rice codon optimized
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cpf1 is rice codon optimized

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ230-RVR was a gift from Yiping Qi (Addgene plasmid # 108861 ; http://n2t.net/addgene:108861 ; RRID:Addgene_108861)

  • For your References section:

    Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Zhong Z, Zhang Y, You Q, Tang X, Ren Q, Liu S, Yang L, Wang Y, Liu X, Liu B, Zhang T, Zheng X, Le Y, Zhang Y, Qi Y. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. 10.1016/j.molp.2018.03.008 PubMed 29567452
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