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PurposeEncodes for the fluorescent protein mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET-B
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesDiscosoma (coral)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET-B mCherry was a gift from Kalina Hristova (Addgene plasmid # 108857 ; http://n2t.net/addgene:108857 ; RRID:Addgene_108857) -
For your References section:
Uninduced high-yield bacterial expression of fluorescent proteins. Sarabipour S, King C, Hristova K. Anal Biochem. 2014 Mar 15;449:155-7. doi: 10.1016/j.ab.2013.12.027. Epub 2013 Dec 28. 10.1016/j.ab.2013.12.027 PubMed 24378720