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PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKDR
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Alt nameFLK1
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Alt nameVEGFR2
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SpeciesH. sapiens (human)
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GenBank ID
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Entrez GeneKDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
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Tag
/ Fusion Protein
- Labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VEGFR2-mCh was a gift from Kalina Hristova (Addgene plasmid # 108854 ; http://n2t.net/addgene:108854 ; RRID:Addgene_108854) -
For your References section:
VEGFR-2 conformational switch in response to ligand binding. Sarabipour S, Ballmer-Hofer K, Hristova K. Elife. 2016 Apr 7;5:e13876. doi: 10.7554/eLife.13876. 10.7554/eLife.13876 PubMed 27052508