VEGFR2-YFP
(Plasmid #108853)

• Purpose: Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker
• Depositing Lab: Kalina Hristova
• Publication: Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554/eLife.13876.

Backbone

• Vector backbone: pBE
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: See Depositor Comments below.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KDR
• Alt name: FLK1
• Alt name: VEGFR2
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
• Tag / Fusion Protein:
  • Labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Restriction digest results suggest that this plasmid may undergo recombination due to the large insert size. We recommend screening at least 3-5 colonies by restriction digest upon receipt.