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Addgene

EphA2-YFP
(Plasmid #108852)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108852 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPHA2
  • Alt name
    ECK
  • Species
    H. sapiens (human)
  • Entrez Gene
    EPHA2 (a.k.a. ARCC2, CTPA, CTPP1, CTRCT6, ECK)
  • Tag / Fusion Protein
    • Labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EphA2-YFP was a gift from Kalina Hristova (Addgene plasmid # 108852 ; http://n2t.net/addgene:108852 ; RRID:Addgene_108852)
  • For your References section:

    EphA2 Receptor Unliganded Dimers Suppress EphA2 Pro-tumorigenic Signaling. Singh DR, Ahmed F, King C, Gupta N, Salotto M, Pasquale EB, Hristova K. J Biol Chem. 2015 Nov 6;290(45):27271-9. doi: 10.1074/jbc.M115.676866. Epub 2015 Sep 11. 10.1074/jbc.M115.676866 PubMed 26363067