-
PurposeEncodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 108852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1+
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEPHA2
-
Alt nameECK
-
SpeciesH. sapiens (human)
-
Entrez GeneEPHA2 (a.k.a. ARCC2, CTPA, CTPP1, CTRCT6, ECK)
-
Tag
/ Fusion Protein
- Labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
EphA2-YFP was a gift from Kalina Hristova (Addgene plasmid # 108852 ; http://n2t.net/addgene:108852 ; RRID:Addgene_108852) -
For your References section:
EphA2 Receptor Unliganded Dimers Suppress EphA2 Pro-tumorigenic Signaling. Singh DR, Ahmed F, King C, Gupta N, Salotto M, Pasquale EB, Hristova K. J Biol Chem. 2015 Nov 6;290(45):27271-9. doi: 10.1074/jbc.M115.676866. Epub 2015 Sep 11. 10.1074/jbc.M115.676866 PubMed 26363067