EcPPK1
(Plasmid #108850)

• Purpose: Expresses E. coli PPK1 in mammalian cell lines
• Depositing Lab: Michael Downey
• Publication: Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439. doi: 10.1016/j.celrep.2018.02.104.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 7472
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Polyphosphate Kinase 1
• Alt name: PPK1
• Species: E. coli
• Insert Size (bp): 2067
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TCCACCACACTGGACTAGTGATGGGTCAGGAAAAGCTATAC
• 3′ sequencing primer: TGATCAGCGGTTTAAACTTATTATTCAGGTTGTTCGAGTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PPK origin is Université de Lausanne
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry