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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMIG
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePP2A Aalpha
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Alt namePP2A
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SpeciesH. sapiens (human)
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MutationE64G
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Entrez GenePPP2R1A (a.k.a. HJS2, MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E64G substitution found in a breast carcinoma.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIG-Aalpha E64G was a gift from William Hahn (Addgene plasmid # 10885 ; http://n2t.net/addgene:10885 ; RRID:Addgene_10885) -
For your References section:
Cancer-associated PP2A Aalpha subunits induce functional haploinsufficiency and tumorigenicity. Chen W, Arroyo JD, Timmons JC, Possemato R, Hahn WC. Cancer Res. 2005 Sep 15. 65(18):8183-92. 10.1158/0008-5472.CAN-05-1103 PubMed 16166293
Map uploaded by the depositor.
