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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
(Plasmid #108770)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108770 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression, Cre/Lox, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flp recombinase fragment aa397 to C-terminus
  • Species
    Synthetic
  • Promoter pCAG
  • Tags / Fusion Proteins
    • PYL (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA was a gift from Wilson Wong (Addgene plasmid # 108770 ; http://n2t.net/addgene:108770 ; RRID:Addgene_108770)

  • For your References section:

    High-performance chemical- and light-inducible recombinases in mammalian cells and mice. Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J, Wong WW. Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7. 10.1038/s41467-019-12800-7 PubMed 31649244
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