pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
(Plasmid
#108730)
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PurposeExpresses aa271 to C-terminus of Cre recombinase fused to GAI CID
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression, Cre/Lox, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase fragment aa271 to C-terminus
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SpeciesSynthetic
- Promoter pCAG
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Tags
/ Fusion Proteins
- GAI (N terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA was a gift from Wilson Wong (Addgene plasmid # 108730 ; http://n2t.net/addgene:108730 ; RRID:Addgene_108730) -
For your References section:
High-performance chemical- and light-inducible recombinases in mammalian cells and mice. Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J, Wong WW. Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7. 10.1038/s41467-019-12800-7 PubMed 31649244