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Addgene

pMpGE006
(Plasmid #108722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMpGWB103
  • Backbone manufacturer
    Kohchi Lab., Kyoto
  • Backbone size w/o insert (bp) 11315
  • Total vector size (bp) 15938
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Atco-Cas9-Pea3ter
  • Species
    Synthetic
  • Insert Size (bp)
    4623
  • Promoter MpEFpro

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CACCATGGATAAGAAGTACTCTATCGG
  • 3′ sequencing primer CAGGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Atco-Cas9-Pea3ter is from Holger Puchta Lab. Fauser et al. (2014) Plant J

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGE006 was a gift from Takayuki Kohchi & Shigeo Sugano (Addgene plasmid # 108722 ; http://n2t.net/addgene:108722 ; RRID:Addgene_108722)

  • For your References section:

    Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. 10.1371/journal.pone.0205117 PubMed 30379827
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