pT2-CAG-fGFP
(Plasmid #108714)

• Purpose: Transposon donor plasmid for long-term expression of membrane targetted GFP
• Depositing Lab: Madeline Lancaster
• Publication: Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666. doi: 10.1038/nbt.3906. Epub 2017 May 31.

Backbone

• Vector backbone: pT2HB
• Backbone manufacturer: Perry Hackett (Addgene plasmid 26557)
• Modifications to backbone: This plasmid was modified so that it included additional restriction sites and attR sites that would allow the insertion of sequences using Gateway cloning.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: Synthetic; Aequorea victoria (Jellyfish)
• Promoter: CAG
• Tags / Fusion Proteins:
  • farnesylation signal (C terminal on insert)
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The source plasmid with GFP was a gift from Safa Lucken-Ardjomande Häsler (member of the Harvey McMahon group at the MRC LMB). Dr. Häsler cloned the gene from the pEGFP-N3 plasmid, originally received from Clontech.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry