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ATG-2460
(Plasmid #108713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-T2A-copGFP
  • Backbone manufacturer
    Systembio
  • Backbone size w/o insert (bp) 6460
  • Total vector size (bp) 8917
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CBR2opt-copGFP fusion
  • Species
    Pyrophorus plagiophthalamus
  • Insert Size (bp)
    2456
  • Mutation
    Promega used directed evolution to create CBR (Click Beetle Red). CBR2 contains 2 additional mutations (R344S+G351R) This gene is also codon optimized for mouse expression
  • Promoter elongation factor-1
  • Tag / Fusion Protein
    • CBR2opt fused to copGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Promega
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATG-2460 was a gift from Keith Wood (Addgene plasmid # 108713 ; http://n2t.net/addgene:108713 ; RRID:Addgene_108713)

  • For your References section:

    Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging. Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi C, Kirkland TA, Encell LP, Wood KV, Lowik C, Mezzanotte L. Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9. 10.1038/s41467-017-02542-9 [pii] PubMed 29317625
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