ATG-1929
(Plasmid #108712)

• Purpose: CBR2opt in pF4Ag expression vector with T7 and CMV promoter
• Depositing Lab: Keith Wood
• Publication: Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9.

Backbone

• Vector backbone: pF4Ag
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3820
• Total vector size (bp): 5449
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBR2opt
• Species: Pyrophorus plagiophthalamus
• Insert Size (bp): 1629
• Mutation: Promega used directed evolution to create CBR (Click Beetle Red). CBR2 contains 2 additional mutations (R344S+G351R) This gene is also codon optimized for mouse expression
• Promoter: T7, CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sgf1 (not destroyed)
• 3′ cloning site: Pme1 (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Promega
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • CBR2 Limited Use Label License
• Industry Terms:
  • Not Available to Industry